Recombinant Danio rerio (zebrafish) VEGFA protein(Met1-Arg188)

• Cat.No.: VEGFA-049Z
• Product Overview: Recombinant Zebrafish VEGF165 (O73682-Isoform VEGF165) (Met 1-Arg188) was expressed and purified in Insect Cells.

Specification

• Source: Insect Cells
• Species: Zebrafish
• Tag: N/A
• Protein length: Met1-Arg188
• Form: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 30-250ng/mL.
• Molecular Mass: The recombinant zebrafish VEGF165 consists of 165 amino acids and has a calculated molecular mass of 19.2 kDa. It migrates as an 22 KDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

04/21/2021

VEGFA's catalytic activity is so strong that it can adapt even in the face of complex chemical reactions.

03/09/2020

VEGFA they produced was highly active and the experimental effect was obvious.

Q&As

How does VEGFA protein relate to other biomarkers?

Studies have shown that there is an association between the expression level of VEGFA protein and other biomarkers, for example, it can be associated with indicators such as tumor cell differentiation, metastatic capacity, and prognosis.

How to harness the activity of VEGFA protein for disease treatment?

At present, a variety of drugs have been developed to target the VEGFA protein and its related signaling pathway, including inhibiting its expression and inhibiting its binding to receptors, etc., for the treatment of tumors and other vascular-related diseases.

What is the role of VEGFA protein in tumor angiogenesis?

VEGFA protein plays a key role in tumor angiogenesis, promoting tumor growth and metastasis by stimulating endothelial cell proliferation, migration, and vascular network formation.

How well is the VEGFA protein expressed in different tissues and organs?

The expression of VEGFA protein in different tissues and organs is different, and it is expressed in tumor tissue, cardiovascular system and other tissues and organs, and the expression level may be affected by many factors.

What is the relationship between the expression level of VEGFA protein and the degree of tumor malignancy?

Studies have shown that the expression level of VEGFA protein is related to the degree of malignancy of the tumor, and the high expression of VEGFA protein often indicates that the tumor is more aggressive.

How about the fuction of VEGFA protein in cardiovascular disease?

VEGFA protein plays an important role in cardiovascular disease by regulating endothelial cell function, promoting vascular repair and maintaining cardiovascular system homeostasis.