Recombinant DENV(type 2, strain New Guinea C/PUO-218 hybrid) E protein(Phe247-Gly675), His-tagged

• Cat.No.: E-2432D
• Product Overview: Recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Phe247-Gly675, with two amino acids "MV" at the N-terminal) was expressed in Insect Cells with a polyhistidine tag at the C-terminus.

Specification

• Species: DENV
• Source: Insect Cells
• Tag: His
• Protein Length: Phe247-Gly675
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 442 amino acids and predicts a molecular mass of 49.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 80 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.