Recombinant Dog CTNNB1 Protein

• Cat.No.: CTNNB1-01D
• Product Overview: Recombinant Dog CTNNB1 Protein without tag was expressed in E. coli.

Specification

• Description: Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
• Source: E. coli
• Species: Dog
• Molecular Mass: 86.5 kDa
• AA Sequence: MHHHHHHHHATQADLMELDMAMEPD RKAAVSHWQQQSYLDSGIHSGATTT APSLSGKGNPEEEDVDTTQVLYEWE QGFSQSFTQEQVADIDGQYAMTRAQ RVRAAMFPETLDEGMQIPSTQFDAA HPTNVQRLAEPSQMLKHAVVNLINY QDDAELATRAIPELTKLLNDEDQVV VNKAAVMVHQLSKKEASRHAIMRSP QMVSAIVRTMQNTNDVETARCTAGT LHNLSHHREGLLAIFKSGGIPALVK MLGSPVDSVLFYAITTLHNLLLHQE GAKMAVRLAGGLQKMVALLNKTNVK FLAITTDCLQILAYGNQESKLIILA SGGPQALVNIMRTYTYEKLLWTTSR VLKVLSVCSSNKPAIVEAGGMQALG LHLTDPSQRLVQNCLWTLRNLSDAA TKQEGMEGLLGTLVQLLGSDDINVV TCAAGILSNLTCNNYKNKMMVCQVG GIEALVRTVLRAGDREDITEPAICA LRHLTSRHQEAEMAQNAVRLHYGLP VVVKLLHPPSHWPLIKATVGLIRNL ALCPANHAPLREQGAIPRLVQLLVR AHQDTQRRTSMGGTQQQFVEGVRME EIVEGCTGALHILARDVHNRIVIRG LNTIPLFVQLLYSPIENIQRVAAGV LCELAQDKEAAEAIEAEGATAPLTE LLHSRNEGVATYAAAVLFRMSEDKP QDYKKRLSVELTSSLFRTEPMAWNE TADLGLDIGAQGEPLGYRQDDPSYR SFHSGGYGQDALGMDPMMEHEMGGH HPGADYPVDGLPDLGHAQDLMDGLP PGDSNQLAWFDTD
• Purity: > 90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 0.56 mg/mL
• Storage Buffer: 50mM Tris, 300mM NaCl, pH 8.0

Gene Information

• Gene Name: CTNNB1 catenin beta 1 [ Canis lupus familiaris (dog) ]
• Official Symbol: CTNNB1
• Synonyms: CTNNB1; catenin beta 1; catenin beta-1; beta-catenin
• Gene ID: 477032
• mRNA Refseq: NM_001137652
• Protein Refseq: NP_001131124
• UniProt ID: B6V8E6