Recombinant E.coli birA, His-MBP tagged

• Cat.No.: birA-339E
• Product Overview: Recombinant E.coli birA (P06709) (Met1-Lys321), fused with an N-terminal polyhistidine-tagged MBP tag, was produced in E. coli.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-321 a.a.
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization.
• Molecular Mass: The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Publications:
  Rapid generation of potent antibodies by autonomous hypermutation in yeast (2021)