Recombinant E.coli birA, His-MBP tagged
Cat.No. : | birA-339E |
Product Overview : | Recombinant E.coli birA (P06709) (Met1-Lys321), fused with an N-terminal polyhistidine-tagged MBP tag, was produced in E. coli. |
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Source : | E. coli |
Species : | E.coli |
Tag : | His-MBP |
Predicted N Terminal : | Met |
Form : | Lyophilized from sterile 50mM Tris, 100mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
Molecular Mass : | The recombinant E.coil BirA/MBP fusion protein consists of 713 amino acids and has a calculated molecular mass of 78.3 kDa. It migrates as an approximately 64-68 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Purity : | >95 % as determined by SDS-PAGE |
Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Publication : |
Rapid generation of potent antibodies by autonomous hypermutation in yeast (2021)
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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