Recombinant EBV BFRF3 Antigen, His tagged, Biotinylated

• Cat.No.: BFRF3-006E
• Product Overview: This recombinant antigen is a truncated form of the capsid protein VP26, the putative 18 kDa (VCA), which is codified by the BFRF3 gene of EBV and monobiotinylated in vivo.

Specification

• Species: EBV
• Source: E.coli
• Tag: His
• Description: p18 is an antigen of 18 kDa codified by the BFRF3 gene. Antibody to VCA are found both in early and late EBV infection. At the time of infection, antibody of both the IgM and IgG types are detectable. After four to six months, usually, only the IgG antibody against VCA can be found. VCA-p18 can be considered to be the best dominant immunoreactive antigen for use in IgM VCA diagnosis.
• Tag: N-His
• Conjugation/Label: Biotin
• Molecular Mass: Determined by SDS-PAGE, the protein band is between the molecular markers of 25 and 35 kDa, while relative molecular mass calculated from amino acid sequence is 23,799.59 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, CE, NP, PO
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7 and 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.06 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Wout M. J. Van Grunsven, Erika C. Van Heerde, Hans J. W. De Haard, Willy J. M. Spaan and Jaap M. Middeldorp. Gene Mapping and Expression of Two Immunodominant Epstein-Barr Virus Capsid Proteins. 1993, J. of Virology, p. 3908-3916.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
• Conjugation: Biotin