Recombinant EBV BZLF1 Antigen, His tagged

• Cat.No.: BZLF1-011E
• Product Overview: The EBV gene BZLF1 has been expressed as a recombinant antigen zebra. It is produced from the complete ORF of the bZIP transcription factor.

Specification

• Species: EBV
• Source: E.coli
• Tag: His
• Description: The protein ZEBRA is a component of the EA complex, also called EB1, which is encoded by the EBV immediate early gen BZLF1. Zebra antibodies are a good marker for detecting the occurrence of EBV reactivation.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is at the molecular marker is between molecular markers of 45 kDa and 35 kDa, while relative molecular mass calculated from amino acid sequence is 35,081.1 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl, 0.1% polyoxyethylene (10) tridecylether and 4 M urea
• Concentration: 1.27 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Yamauchi Y, Y. Tachiband, A. Maeda, H. Wakiguchi, M. Usui, T. Kurata and T. Sairenji. Evaluation of antibodies to the Epstein-Barr virus immediate early gene product ZEBRA by a new enzyme-linked immunosorbent assay. 1998, Intervirology, 41:278-284.
  2. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.