Recombinant EBV chimeric viral capsid antigen, His&TrxA tagged

• Cat.No.: VCA-085E
• Product Overview: The recombinant chimeric viral capsid antigen ChimEBV-VCA for Epstein-Barr Virus (EBV) has been prepared as a recombinant multi-epitope antigen fused to a his-tag and trxA at the N-terminal.

Specification

• Species: EBV
• Source: E.coli
• Tag: His&TRxA
• Tag: N-His&TrxA
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 45-66.2 kDa, while relative molecular mass calculated from amino acid sequence is 43,041.44 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.06 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Wout M. J. Van Grunsven, Erika C. Van Heerde, Hans J. W. De Haard, Willy J. M. Spaan and Jaap M. Middeldorp. Gene Mapping and Expression of Two Immunodominant Epstein-Barr Virus Capsid Proteins. 1993, J. of Virology, p. 3908-3916.
  2. Färber, I., Hinderer, W., Rothe, M. Lang, D., Sonneborn, H.H. and Wutzler, P. Serological diagnosis of Epstein-Barr virus infection by novel ELISAs based on recombinant capsid antigens p23 and p18. 2001, J. Med. Virol, 63: 271-6.
  3. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.