Recombinant E.coli can Protein, His-tagged can-395E

Recombinant E.coli can Protein, His-tagged

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Recombinant E.coli can Protein, His-tagged

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Cat.No. : can-395E
Product Overview : Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques.
Description : Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O<->HCO3- + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life.
Source : E.coli
Species : E.coli
Tag : His
Form : Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
Bio-activity : Specific activity is >1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
Molecular Mass : 27kDa (240aa), confirmed by MALDI-TOF
AA Sequence : MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK
Purity : >95% by SDS - PAGE
Storage : Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 1 mg/ml (determined by Bradford assay)
Gene Name : can carbonic anhydrase [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : can
Synonyms : ECK0125; JW0122; yadF; carbonic anhydrase; putative carbonic anhdrase
Gene ID : 944832
mRNA Refseq :
Protein Refseq : NP_414668
MIM :
UniProt ID : P61517
Pathway : Nitrogen metabolism, organism-specific biosystem; Nitrogen metabolism, conserved biosystem

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