Recombinant Ehrlichia canis gp19 Antigen, His tagged

• Cat.No.: gp19-025E
• Product Overview: The gp19 of E. canis has been prepared as a recombinant truncated antigen containing specific antigenic determinants described in the bibliography and fused to a his-tag in its N-terminal.

Specification

• Species: Ehrlichia canis
• Source: E.coli
• Tag: His
• Description: gp19 is a major epitope of the bacteria Ehrlichia canis, which can be used for the detection of early acute-phase antibodies and reacts strongly with antibodies in late-acute-phase sera from experimental dogs. gp19 is encoded by a highly conserved single gene that minimizes or eliminates the potential for serologic variability.
• Tag: N-His
• Molecular Mass: Determined by SDS-PAGE, the protein band is between molecular markers of 35-25 kDa, while relative molecular mass calculated from amino acid sequence is 21.386,62 Da.
• Purity: > 95% by observation on SDS-PAGE electrophoresis
• Applications: WB, DB, IE, DE, CLIA, LF
• Storage: Upon arrival, it should be aliquoted to avoid repeated freezing and thawing cycles and stored at -20 to -80 centigrade. In order to defrost the protein, maintain the aliquot at 25 centigrade without shaking to avoid aggregation.
• Storage Buffer: 20 mM phosphate buffer pH 7, 0.15 M NaCl, 0.13 M trehalose and 0.1% polyoxyethylene (10) tridecyl ether
• Concentration: 1.26 mg/mL
• Shipping: Protein is shipped with dry ice.
• Reference: 1. Gill SC, von Hippel PH. Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem. 1989 Nov 1;182(2):319-26.
  2. McBride, J.W., Doyle, C.K., Zhang, X., Cardenas, A.M., Popov, V.L., Nethery, K.A. and M. E. Woods. Identification of a Glycosylated Ehrlichia canis 19-Kilodalton Major Immunoreactive Protein with a Species-Specific Serine-Rich Glycopeptide Epitope. 2007. Infection and Immunity, 1 (75): 74-82.