Recombinant Escherichia coli GADA Protein (1-466 aa), His-tagged

• Cat.No.: GADA-859E
• Product Overview: Recombinant Escherichia coli (strain K12) GADA Protein (1-466 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.

Specification

• Description: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad syst helps to maintain a near-neutral intracellular pH when cells are exposed to extrely acidic conditions. The ability to survive transit through the acidic conditions of the stomach is essential for successful colonization of the mammalian host by commensal and pathogenic bacteria.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 56.7 kDa
• Protein length: 1-466 aa
• AA Sequence: MDQKLLTDFRSELLDSRFGAKAIST IAESKRFPLHEMRDDVAFQIINDEL YLDGNARQNLATFCQTWDDENVHKL MDLSINKNWIDKEEYPQSAAIDLRC VNMVADLWHAPAPKNGQAVGTNTIG SSEACMLGGMAMKWRWRKRMEAAGK PTDKPNLVCGPVQICWHKFARYWDV ELREIPMRPGQLFMDPKRMIEACDE NTIGVVPTFGVTYTGNYEFPQPLHD ALDKFQADTGIDIDMHIDAASGGFL APFVAPDIVWDFRLPRVKSISASGH KFGLAPLGCGWVIWRDEEALPQELV FNVDYLGGQIGTFAINFSRPAGQVI AQYYEFLRLGREGYTKVQNASYQVA AYLADEIAKLGPYEFICTGRPDEGI PAVCFKLKDGEDPGYTLYDLSERLR LRGWQVPAFTLGGEATDIVVMRIMC RRGFEMDFAELLLEDYKASLKYLSD HPKLQGIAQQNSFKHT
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.
• UniProt ID: P69908

Gene Information

• UniProt ID: P69908