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Recombinant Escherichia coli LIGA Protein (1-671 aa), His-tagged

Cat.No. : LIGA-886E
Product Overview : Recombinant Escherichia coli (strain K12) LIGA Protein (1-671 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length.
  • Specification
  • Gene Information
  • Related Products
Description : DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.
Source : E. coli
Species : E. coli
Tag : His
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 77.6 kDa
Protein length : 1-671 aa
AA Sequence : MESIEQQLTELRTTLRHHEYLYHVM DAPEIPDAEYDRLMRELRELETKHP ELITPDSPTQRVGAAPLAAFSQIRH EVPMLSLDNVFDEESFLAFNKRVQD RLKNNEKVTWCCELKLDGLAVSILY ENGVLVSAATRGDGTTGEDITSNVR TIRAIPLKLHGENIPARLEVRGEVF LPQAGFEKINEDARRTGGKVFANPR NAAAGSLRQLDPRITAKRPLTFFCY GVGVLEGGELPDTHLGRLLQFKKWG LPVSDRVTLCESAEEVLAFYHKVEE DRPTLGFDIDGVVIKVNSLAQQEQL GFVARAPRWAVAFKFPAQEQMTFVR DVEFQVGRTGAITPVARLEPVHVAG VLVSNATLHNADEIERLGLRIGDKV VIRRAGDVIPQVVNVVLSERPEDTR EVVFPTHCPVCGSDVERVEGEAVAR CTGGLICGAQRKESLKHFVSRRAMD VDGMGDKIIDQLVEKEYVHTPADLF KLTAGKLTGLERMGPKSAQNVVNAL EKAKETTFARFLYALGIREVGEATA AGLAAYFGTLEALEAASIEELQKVP DVGIVVASHVHNFFAEESNRNVISE LLAEGVHWPAPIVINAEEIDSPFAG KTVVLTGSLSQMSRDDAKARLVELG AKVAGSVSKKTDLVIAGEAAGSKLA KAQELGIEVIDEAEMLRLLGS
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
UniProt ID : P15042
UniProt ID : P15042

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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