Recombinant Escherichia coli MRCA Protein (229-529 aa), His-SUMO-tagged

• Cat.No.: MRCA-2110E
• Product Overview: Recombinant Escherichia coli (strain K12) MRCA Protein (229-529 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Microbiology. Protein Description: Partial.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His&SUMO
• Protein Length: 229-529 aa
• Description: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 49.5 kDa
• AA Sequence: MLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRYGESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNNKITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQGPTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQSKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDASAGSDWQPKNSPPQYAGPIRLRQGLGQSKNVVM
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: mrcA peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: MRCA
• Synonyms: ECK3383; ponA
• Gene ID: 947907
• Protein Refseq: NP_417855
• UniProt ID: P02918