Recombinant Escherichia coli MRCA Protein (229-529 aa), His-SUMO-tagged

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Recombinant Escherichia coli MRCA Protein (229-529 aa), His-SUMO-tagged

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Cat.No. : MRCA-2110E
Product Overview : Recombinant Escherichia coli (strain K12) MRCA Protein (229-529 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Microbiology. Protein Description: Partial.
Description : Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits).
Source : E. coli
Species : E. coli
Tag : His-SUMO
Form : Tris-based buffer,50% glycerol
Molecular Mass : 49.5 kDa
Protein length : 229-529 aa
AA Sequence : MLDEGYITQQ QFDQTRTEAI NANYHAPEIA FSAPYLSEMV RQEMYNRYGE SAYEDGYRIY TTITRKVQQA AQQAVRNNVL DYDMRHGYRG PANVLWKVGE SAWDNNKITD TLKALPTYGP LLPAAVTSAN PQQATAMLAD GSTVALSMEG VRWARPYRSD TQQGPTPRKV TDVLQTGQQI WVRQVGDAWW LAQVPEVNSA LVSINPQNGA VMALVGGFDF NQSKFNRATQ ALRQVGSNIK PFLYTAAMDK GLTLASMLND VPISRWDASA GSDWQPKNSP PQYAGPIRLR QGLGQSKNVV M
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with reconstitution instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : mrcA peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA [ Escherichia coli str. K-12 substr. MG1655 ]
Official Symbol : MRCA
Synonyms : ECK3383; ponA;
Gene ID : 947907
Protein Refseq : NP_417855
UniProt ID : P02918

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