Recombinant Escherichia coli O157:H7 recG protein, His-SUMO-tagged

• Cat.No.: recG-4514E
• Product Overview: Recombinant Escherichia coli O157:H7 recG protein(Q8XD86)(1-693aa), fused to N-terminal His tag and SUMO tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Escherichia coli O157:H7
• Tag: His-SUMO
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 89.4 kDa
• Protein length: 1-693aa
• AA Sequence: MTGRLLDAVPLSSLTGVGAALSNKL AKINLHTVQDLLLHLPLRYEDRTHL YPIGELLPGVYATVEGEVLNCNISF GGRRMMTCQISDGSGILTMRFFNFS AAMKNSLATGRRVLAYGEAKRGKYG AEMIHPEYRVQGDLSTPELQETLTP VYPTTEGVKQATLRKLTDQALDLLD TCAIEELLPPELSQGMMTLPEALRT LHRPPPTLQLSDLETGQHPAQRRLI LEELLAHNLSMLALRAGAQRFHAQP LSANDALKNKLLAALPFKPTGAQAR VVAEIERDMALDVPMMRLVQGDVGS GKTLVAALAALRAIAHGKQVALMAP TELLAEQHANNFRNWFEPLGIEVGW LAGKQKGKARLSQQEAIASGQVQMI VGTHAIFQEQVQFNGLALVIIDEQH RFGVHQRLALWEKGQQQGFHPHQLI MTATPIPRTLAMTAYADLDTSVIDE LPPGRTPVTTVAIPDTRRTDIIDRV RHACITEGRQAYWVCTLIEESELLE AQAAEATWEELKLALPELNVGLVHG RMKPAEKQAVMASFKQGELHLLVAT TVIEVGVDVPNASLMIIENPERLGL AQLHQLRGRVGRGAVASHCVLLYKT PLSKTAQIRLQVLRDSNDGFVIAQK DLEIRGPGELLGTRQTGNAEFKVAD LLRDQAMIPEVQRLARHIHERYPQQ AKALIERWMPETERYSNA
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.