Recombinant Escherichia coli RPSG Protein (30S) (2-179 aa), His-tagged

• Cat.No.: RPSG-1224E
• Product Overview: Recombinant Escherichia coli (strain K12) RPSG Protein (30S) (2-179 aa) is produced by E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assbly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center, where it has been shown to contact mRNA. Has been shown to contact tRNA in both the P and E sites; it probably blocks exit of the E site tRNA.
• Source: E. coli
• Species: E. coli
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 23.9 kDa
• Protein length: 2-179 aa
• AA Sequence: PRRRVIGQRKILPDPKFGSELLAKF VNILMVDGKKSTAESIVYSALETLA QRSGKSELEAFEVALENVRPTVEVK SRRVGGSTYQVPVEVRPVRRNALAM RWIVEAARKRGDKSMALRLANELSD AAENKGTAVKKREDVHRMAEANKAF AHYRWLSLRSFSHQAGASSKQPALG YLN
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: rpsG 30S ribosomal subunit protein S7 [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: RPSG
• Synonyms: ECK3328
• Gene ID: 947846
• Protein Refseq: NP_417800
• UniProt ID: P02359