Recombinant Escherichia coli (strain K12) pykF protein, His-tagged

• Cat.No.: pykF-4512E
• Product Overview: Recombinant Escherichia coli (strain K12) pykF protein(P0AD61)(1-470aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Escherichia coli (strain K12)
• Tag: His
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 54.8 kDa
• Protein length: 1-470aa
• AA Sequence: MKKTKIVCTIGPKTESEEMLAKMLD AGMNVMRLNFSHGDYAEHGQRIQNL RNVMSKTGKTAAILLDTKGPEIRTM KLEGGNDVSLKAGQTFTFTTDKSVI GNSEMVAVTYEGFTTDLSVGNTVLV DDGLIGMEVTAIEGNKVICKVLNNG DLGENKGVNLPGVSIALPALAEKDK QDLIFGCEQGVDFVAASFIRKRSDV IEIREHLKAHGGENIHIISKIENQE GLNNFDEILEASDGIMVARGDLGVE IPVEEVIFAQKMMIEKCIRARKVVI TATQMLDSMIKNPRPTRAEAGDVAN AILDGTDAVMLSGESAKGKYPLEAV SIMATICERTDRVMNSRLEFNNDNR KLRITEAVCRGAVETAEKLDAPLIV VATQGGKSARAVRKYFPDATILALT TNEKTAHQLVLSKGVVPQLVKEITS TDDFYRLGKELALQSGLAHKGDVVV MVSGALVPSGTTNTASVHVL
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.