Recombinant Escherichia coli (strain UTI89/UPEC) clpP protein (ATP-dependent Clp protease proteolytic subunit), His/Myc-tagged

• Cat.No.: CLPP-1032E
• Product Overview: Recombinant Escherichia coli (strain UTI89/UPEC) clpP protein (ATP-dependent Clp protease proteolytic subunit, 15-207aa), fused to 10×His-tag at N-terminus and Myc-tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: ClpP can simultaneously bind ClpX and ClpA to form a heteromeric complex. ClpP is a serine protease with a chymotrypsin-like activity that is a part of the ClpAP, ClpAPX and ClpXP protease complexes. Cleaves peptides in various proteins in a process that requires ATP hydrolysis. The protein has a chymotrypsin-like activity and plays a major role in the degradation of misfolded proteins.
• Source: E.coli
• Species: E.coli
• Tag: His/Myc
• Form: Tris-based buffer，50% glycerol.
• Molecular Mass: 28.6 kDa
• Protein length: 193
• AA Sequence: ALVPMVIEQTSRGERSFDIYSRLLK ERVIFLTGQVEDHMANLIVAQMLFL EAENPEKDIYLYINSPGGVITAGMS IYDTMQFIKPDVSTICMGQAASMGA FLLTAGAKGKRFCLPNSRVMIHQPL GGYQGQATDIEIHAREILKVKGRMN ELMALHTGQSLEQIERDTERDRFLS APEAVEYGLVDSILTHRN
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20/-80 centigrade. The shelf life of lyophilized form is 12 months at -20/-80 centigrade. Store working aliquots at 4 centigrade for up to one week.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Warning: Repeated freezing and thawing is not recommended.
• Synonyms: ECK0431; lopP
• UniProt ID: Q1RF98

Gene Information

• Synonyms: ECK0431; lopP
• UniProt ID: Q1RF98