Recombinant Escherichia phage T5 (Enterobacteria phage T5) D15 Protein (Met1-Gln291), N-His tagged

• Cat.No.: D15-1629E
• Product Overview: Recombinant Enterobacteria phage T5 D15 (Met1-Gln291) fused with the N-His tag was expressed in E. coli.

Specification

• Species: Escherichia phage T5 (Enterobacteria phage T5)
• Source: E.coli
• Tag: His
• Protein Length: Met1-Gln291
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 35.62 kDa
• Purity: >90% as determined by SDS-PAGE.
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.
  Store at 2 to 8 centigrade for one week.
  Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in 50 mM KAc, 20 mM Tris-Ac, 10 mM Mg (Ac)2, 1 mM DTT (pH 7.9 at 25 centigrade) or lyophilized from 50 mM KAc, 20 mM Tris-Ac, 10 mM Mg (Ac)2, 1 mM DTT (pH 7.9 at 25 centigrade).
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: D15 flap endonuclease [ Escherichia phage T5 ]
• Official Symbol: D15
• Synonyms: D15; flap endonuclease; flap endonuclease; flap endonucleases, or 5'-nucleases, are involved in DNA replication and repair; they possess both 5'-exonucleolytic activity and the ability to cleave bifurcated, or branched DNA, in an endonucleolytic structure-specific manner; 5'-nuclease D15 is also required for late transcription; matches to InterPro family IPR002421 and InterPro domains IPR000513 and IPR008918
• Gene ID: 2777611
• Protein Refseq: YP_006958
• UniProt ID: P06229