Recombinant Feline CCL5 Protein

• Cat.No.: CCL5-427F
• Product Overview: Recombinant Feline CCL5 Protein was expressed in E. coli.

Specification

• Description: CCL5, also known as RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), is an 8 kDa beta -chemokine that plays a primary role in the inflammatory immune response by means of its ability to attract and activate leukocytes. Human and mouse RANTES exhibit cross-species activity on human and mouse cells. Mature feline CCL5 shares 84% aa seqeuence identity with canine CCL5 and 71% - 75% with cotton rat, human, mouse, and rat CCL5. CCL5 is secreted by many cell types at inflammatory sites, and it exerts a wide range of activities through the receptors CCR1, CCR3, CCR4, and CCR5. Inflammatory responses can be impaired by the sequestration of CCL5 by the cytomegalovirus protein US28. In humans, CCR5 binding to CCL5 inhibits the infectivity of R5 (M-tropic) but not X4 (T-tropic) strains of HIV-1. The two N-terminal residues of CCL5 can be removed by CD26/DPPIV, generating a protein that functions as a chemotaxis inhibitor and more effectively blocks M-tropic HIV-1 infection of monocytes. Oligomerization of CCL5 on glycosaminoglycans is required for CCR1-mediated leukocyte adhesion and activation as well as CCL5's interaction with the chemokine CXCL4/PF4. The deposition of CCL5 on activated vascular endothelial cells is crucial for monocyte adhesion to damaged vasculature, but CCL5 oligomerization is not required for the extravasation of adherent leukocytes. CCL5 is upregulated in breast cancer and promotes tumor progression through the attraction of proinflammatory macrophages in addition to its actions on tumor cells, stromal cells, and the vasculature.
• Source: E. coli
• Species: Feline
• Protein length: Tyr26-Asn92 (Lys84Tyr and Gly85 del)
• Form: Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
• Bio-activity: Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CCR5. The ED50 for this effect is 3.5-20 ng/mL.
• Molecular Mass: 7.7 kDa
• Endotoxin: <0.01 EU per 1 μg of the protein by the LAL method.
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
• Publication:
  Recruitment of classical monocytes can be inhibited by disturbing heteromers of neutrophil HNP1 and platelet CCL5 (2015)

Gene Information

• Gene Name: CCL5 C-C motif chemokine ligand 5 [ Felis catus (domestic cat) ]
• Official Symbol: CCL5
• Synonyms: CCL5; C-C motif chemokine ligand 5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
• Gene ID: 493689
• mRNA Refseq: NM_001009827.1
• Protein Refseq: NP_001009827.1
• UniProt ID: Q8SQ40