Recombinant Full Length Acanthamoeba Castellanii Atp Synthase Subunit A(Atp6) Protein, His-Tagged

• Cat.No.: RFL9001AF
• Product Overview: Recombinant Full Length Acanthamoeba castellanii ATP synthase subunit a(ATP6) Protein (Q37385) (1-247aa), fused to N-terminal His tag, was expressed in E. coli.

Specification

• Source: E.coli expression system
• Species: Acanthamoeba castellanii (Amoeba)
• Tag: His
• Form: Lyophilized powder
• Protein Length: Full Length (1-247)
• AA Sequence: MIFNPLEQFRISVLQKLFFGNIDIS ITNNTIILFVILIGFTFLFYVNYST NTYIPSKWQY AVENIYLFVLQLFKQQINNIVALKY FPLVLFVFSFILFANLIGLLPYGFT ITGHIIFTFQ IAFSLFFGITLINFFNNKTEFFNLF VPSGVPKPLIPFLVVIEVVSYLIRP FSLSVRLFAN MLAGHTLLNILSAFIFNVFKKYALI SFLPLLFIVFIIVLEFCIAIVQAYI FSILTCIYLN DIYNTSH
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Tris/PBS-based buffer, 6% Trehalose, pH 8.0
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Gene Name: ATP6
• Synonyms: ATP6; ATP synthase subunit a; F-ATPase protein 6
• UniProt ID: Q37385

Gene Information

• Gene Name: ATP6
• Synonyms: ATP6; ATP synthase subunit a; F-ATPase protein 6
• UniProt ID: Q37385

Reviews

01/14/2022

Such ATP6 are well suited for use in our laboratory because they can be stored for a long time and maintain their original properties.

06/25/2021

Due to the high purity of ATP6, the interference of impurities in the experiment can be reduced and the accuracy of the experiment can be improved.

05/30/2021

The sensitivity of ATP6 in Western blot was very high and low concentrations of the target protein could be detected.

Q&As

How to determine the function and activity of ATP6?

In vitro and in vivo experimental techniques, such as patch clamp techniques, enzyme activity assays, etc. can be used to assess the function and activity of ATP6.

How to study the association between ATP6 and specific diseases?

Associations between ATP6 and specific diseases can be explored by studying human tissue samples, cell or animal models, and genetic analysis of patients with related diseases.

In which cell types is ATP6 expressed?

This is expressed in a variety of cell types, especially in muscle and nerve tissues with active metabolism.

How does ATP6 interact with other mitochondrial proteins?

ATP6 may interact with other mitochondrial proteins such as subunits in the electron transport chain to form functional complexes.

What are the physiological effects of deletion or mutation of ATP6 protein?

Deletion or mutation of ATP6 protein may lead to physiological effects such as mitochondrial dysfunction and energy metabolism disorders.

Is the function of ATP6 affected by regulatory mechanisms?

Yes, the function and activity of ATP6 may be regulated by a variety of regulatory mechanisms, including protein phosphorylation, regulation of gene expression, etc.