Recombinant Full Length Human ALKBH1 Protein, C-Flag-tagged

• Cat.No.: ALKBH1-223HFL
• Product Overview: Recombinant Full Length Human ALKBH1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 43.7 kDa
• AA Sequence: MGKMAAAVGSVATLATEPGEDAFRK LFRFYRQSRPGTADLEGVIDFSAAH AARGKGPGAQKVIKSQLNVS SVSEQNAYRAGLQPVSKWQAYGLKG YPGFIFIPNPFLPGYQWHWVKQCLK LYSQKPNVCNLDKHMSKEET QDLWEQSKEFLRYKEATKRRPRSLL EKLRWVTVGYHYNWDSKKYSADHYT PFPSDLGFLSEQVAAACGFE DFRAEAGILNYYRLDSTLGIHVDRS ELDHSKPLLSFSFGQSAIFLLGGLQ RDEAPTAMFMHSGDIMIMSG FSRLLNHAVPRVLPNPEGEGLPHCL EAPLPAVLPRDSMVEPCSMEDWQVC ASYLKTARVNMTVRQVLATD
  QNFPLEPIEDEKRDISTEGFCHLDD QNSEVKRARINPHSTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: ALKBH1 alkB homolog 1, histone H2A dioxygenase [ Homo sapiens (human) ]
• Official Symbol: ALKBH1
• Synonyms: ABH; ABH1; alkB; hABH; ALKBH
• Gene ID: 8846
• mRNA Refseq: NM_006020.3
• Protein Refseq: NP_006011.2
• MIM: 605345
• UniProt ID: Q13686