Recombinant Full Length Human ANAPC5 Protein, C-Flag-tagged

• Cat.No.: ANAPC5-628HFL
• Product Overview: Recombinant Full Length Human ANAPC5 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 84.9 kDa
• AA Sequence: MASVHESLYFNPMMTNGVVHANVFG IKDWVTPYKIAVLVLLNEMSRTGEG AVSLMERRRLNQLLLPLLQG PDITLSKLYKLIEESCPQLANSVQI RIKLMAEGELKDMEQFFDDLSDSFS GTEPEVHKTSVVGLFLRHMI LAYSKLSFSQVFKLYTALQQYFQNG EKKTVEDADMELTSRDEGERKMEKE ELDVSVREEEVSCSGPLSQK QAEFFLSQQASLLKNDETKALTPAS LQKELNNLLKFNPDFAEAHYLSYLN NLRVQDVFSSTHSLLHYFDR LILTGAESKSNGEEGYGRSLRYAAL NLAALHCRFGHYQQAELALQEAIRI AQESNDHVCLQHCLSWLYVL GQKRSDSYVLLEHSVKKAVHFGLPY LASLGIQSLVQQRAFAGKTANKLMD ALKDSDLLHWKHSLSELIDI SIAQKTAIWRLYGRSTMALQQAQML LSMNSLEAVNAGVQQNNTESFAVAL CHLAELHAEQGCFAAASEVL KHLKERFPPNSQHAQLWMLCDQKIQ FDRAMNDGKYHLADSLVTGITALNS IEGVYRKAVVLQAQNQMSEA HKLLQKLLVHCQKLKNTEMVISVLL SVAELYWRSSSPTIALPMLLQALAL SKEYRLQYLASETVLNLAFA QLILGIPEQALSLLHMAIEPILADG AILDKGRAMFLVAKCQVASAASYDQ PKKAEALEAAIENLNEAKNY
  FAKVDCKERIRDVVYFQARLYHTLG KTQERNRCAMLFRQLHQELPSHGVP LINHLTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation, Ubiquitin mediated proteolysis

Gene Information

• Gene Name: ANAPC5 anaphase promoting complex subunit 5 [ Homo sapiens (human) ]
• Official Symbol: ANAPC5
• Synonyms: APC5
• Gene ID: 51433
• mRNA Refseq: NM_016237.5
• Protein Refseq: NP_057321.2
• MIM: 606948
• UniProt ID: Q9UJX4