Recombinant Full Length Human APLNR Protein, C-Flag-tagged

• Cat.No.: APLNR-596HFL
• Product Overview: Recombinant Full Length Human APLNR Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.5 kDa
• AA Sequence: MEEGGDFDNYYGADNQSECEYTDWK SSGALIPAIYMLVFLLGTTGNGLVL WTVFRSSREKRRSADIFIAS LAVADLTFVVTLPLWATYTYRDYDW PFGTFFCKLSSYLIFVNMYASVFCL TGLSFDRYLAIVRPVANARL RLRVSGAVATAVLWVLAALLAMPVM VLRTTGDLENTTKVQCYMDYSMVAT VSSEWAWEVGLGVSSTTVGF VVPFTIMLTCYFFIAQTIAGHFRKE RIEGLRKRRRLLSIIVVLVVTFALC WMPYHLVKTLYMLGSLLHWP CDFDLFLMNIFPYCTCISYVNSCLN PFLYAFFDPRFRQACTSMLCCGQSR CAGTSHSSSGEKSASYSSGH
  SQGPGPNMGKGGEQMHEKSIPYSQE TLVVDTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction

Gene Information

• Gene Name: APLNR apelin receptor [ Homo sapiens (human) ]
• Official Symbol: APLNR
• Synonyms: APJ; APJR; HG11; AGTRL1
• Gene ID: 187
• mRNA Refseq: NM_005161.6
• Protein Refseq: NP_005152.1
• MIM: 600052
• UniProt ID: P35414