Recombinant Full Length Human ARFGAP1 Protein, GST-tagged

• Cat.No.: ARFGAP1-1239HF
• Product Overview: Human ARFGAP1 full-length ORF ( AAH28233, 1 a.a. - 414 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 71.28 kDa
• Protein Length: 414 amino acids
• AA Sequence: MASPRTRKVL KEVRVQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLESQ EDYDPCWSLQ EKYNSRAAAL FRDKVVALAE GREWSLESSP AQNWTPPQPR TLPSMVHRVS GQPQSVTASS DKAFEDWLND DLGSYQGAQG NRYVGFGNTP PPQKKEDDFL NNAMSSLYSG WSSFTTGASR FASAAKEGAT KFGSQASQKF WGHKQQPEPA SELGHSLNEN VLKPAQEKVK EGKIFDDVSS GVSQLASKGV GSKGWRDVTT FFSGKAEGPL DSPSEGHSYQ NSGLDHFQNS NIDQSFWETF GSAEPTKTRK SPSSDSWTCA DTSTERRSSD SWEVWGSAST NRNSNSDGGE GGEGTKKAVP PAVPTDDGWD NQNW
• Applications: Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: ARFGAP1 ADP-ribosylation factor GTPase activating protein 1 [ Homo sapiens ]
• Official Symbol: ARFGAP1
• Synonyms: ARFGAP1; ADP-ribosylation factor GTPase activating protein 1; ADP ribosylation factor 1 GTPase activating protein , ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; bA261N11.3; FLJ10767; ARF1 GAP; ARF GAP 1; GAP protein; ARF1-directed GTPase-activating protein; ADP-ribosylation factor 1 GTPase activating protein; ADP-ribosylation factor 1 GTPase-activating protein; ARF1GAP; HRIHFB2281; MGC39924
• Gene ID: 55738
• mRNA Refseq: NM_018209
• Protein Refseq: NP_060679
• MIM: 608377
• UniProt ID: Q8N6T3