Recombinant Full Length Human ARHGEF11 Protein, C-Flag-tagged

• Cat.No.: ARHGEF11-884HFL
• Product Overview: Recombinant Full Length Human ARHGEF11 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 172.1 kDa
• AA Sequence: MSVRLPQSIDRLSSLSSLGDSAPER KSPSHHRQPSDASETTGLVQRCVII QKDQHGFGFTVSGDRIVLVQ SVRPGGAAMKAGVKEGDRIIKVNGT MVTNSSHLEVVKLIKSGAYVALTLL GSSPSSMGISGLQQDPSPAG APRITSVIPSPPPPPPLPPPQRITG PKPLQDPEVQKHATQILRNMLRQEE KELQRICEVYSRNPASLLEE QIEGARRRVTQLQLKIQQETGGSVD ILPLYGDTSQRPSEGRLSLDSQEGD SGLDSGTERFPSLSESLMNR NSVLSDPGLDSPRTSPVIMARVAQH HRRQGSDAAVPSTGDQGVDQSPKPL IIGPEEDYDPGYFNNESDII FQDLEKLKSRPAHLGVFLRYIFSQA DPSPLLFYLCAEVYQQASPKDSRSL GKDIWNIFLEKNAPLRVKIP EMLQAEIDSRLRNSEDARGVLCEAQ EAAMPEIQEQIHDYRTKRTLGLGSL YGENDLLDLDGDPLRERQVA EKQLAALGDILSKYEEDRSAPMDFA LNTYMSHAGIRLREARPSNTAEKAQ SAPDKDKWLPFFPKTKKSSN SKKEKDALEDKKRNPILKYIGKPKS SSQSTFHIPLSPVEVKPGNVRNIIQ HFENNQQYDAPEPGTQRLST GSFPEDLLESDSSRSEIRLGRSESL KGREEMKRSRKAENVPRSRSDVDMD AAAEATRLHQSASSSTSSLS TRSLENPTPPFTPKMGRRSIESPSL GFCTDTLLPHLLEDDLGQLSDLEPE PDAQNWQHTVGKDVVAGLTQ REIDRQEVINELFVTEASHLRTLRV LDLIFYQRMKKENLMPREELARLFP NLPELIEIHNSWCEAMKKLR EEGPIIKEISDLMLARFDGPAREEL QQVAAQFCSYQSIALELIKTKQRKE SRFQLFMQEAESHPQCRRLQ LRDLIISEMQRLTKYPLLLESIIKH TEGGTSEHEKLCRARDQCREILKYV NEAVKQTENRHRLEGYQKRL DATALERASNPLAAEFKSLDLTTRK MIHEGPLTWRISKDKTLDLHVLLLE DLLVLLQKQDEKLLLKCHSK TAVGSSDSKQTFSPVLKLNAVLIRS VATDKRAFFIICTSKLGPPQIYELV ALTSSDKNTWMELLEEAVRN ATRHPGAAPMPVHPPPPGPREPAQQ GPTPSRVELDDSDVFHGEPEPEELP GGTGSQQRVQGKHQVLLEDP EQEGSAEEEELGVLPCPSTSLDGEN RGIRTRNPIHLAFPGPLFMEGLADS ALEDVENLRHLILWSLLPGH TMETQAAQEPEDDLTPTPSVISVTS HPWDPGSPGQAPPGGEGDNTQLAGL EGERPEQEDMGLCSLEHLPP RTRNSGIWESPELDRNLAEDASSTE AAGGYKVVRKAEVAGSKVVPALPES GQSEPGPPEVEGGTKATGNC FYVSMPSGPPDSSTDHSEAPMSPPQ PDSLPAGQTEPQPQLQGGNDDPRRP SRSPPSLALRDVGMIFHTIE QLTLKLNRLKDMELAHRELLKSLGG ESSGGTTPVGSFHTEAARWTDGSLS PPAKEPLASDSRNSHELGPC
  PEDGSDAPLEDSTADAAASPGPTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Vascular smooth muscle contraction

Gene Information

• Gene Name: ARHGEF11 Rho guanine nucleotide exchange factor 11 [ Homo sapiens (human) ]
• Official Symbol: ARHGEF11
• Synonyms: GTRAP48; PDZ-RHOGEF
• Gene ID: 9826
• mRNA Refseq: NM_198236.3
• Protein Refseq: NP_937879.1
• MIM: 605708
• UniProt ID: O15085