Recombinant Full Length Human ARHGEF9 Protein, Flag tagged

• Cat.No.: ARHGEF9-12HFL
• Product Overview: Recombinant protein of human Cdc42 guanine nucleotide exchange factor (GEF) 9 (ARHGEF9) with Flag tag was expressed in HEK293T.

Specification

• Species: Human
• Source: HEK293T
• Tag: Flag
• Protein Length: 1-516 aa
• Description: The protein encoded by this gene is a Rho-like GTPase that switches between the active (GTP-bound) state and inactive (GDP-bound) state to regulate CDC42 and other genes. This brain-specific protein also acts as an adaptor protein for the recruitment of gephyrin and together these proteins facilitate receceptor recruitement in GABAnergic and glycinergic synapses. Defects in this gene are the cause of startle disease with epilepsy (STHEE), also known as hyperekplexia with epilepsy, as well as several other types of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene.
• AASequence: MTLLITGDSIVSAEAVWDHVTMANRELAFKAGDVIKVLDASNKDWWWGQIDDEEGWFPASFVRLWVNQEDEVEEGPSDVQNGHLDPNSDCLCLGRPLQNRDQMRANVINEIMSTERHYIKHLKDICEGYLKQCRKRRDMFSDEQLKVIFGNIEDIYRFQMGFVRDLEKQYNNDDPHLSEIGPCFLEHQDGFWIYSEYCNNHLDACMELSKLMKDSRYQHFFEACRLLQQMIDIAIDGFLLTLVQKICKYPLQLAELLKYTAQDHSDYRYVAAALAVMRNVTQQINERKRRLENIDKIAQWQASVLDWEGEDILDRSSELIYTGEMAWIYQPYGRNQQRVFFLFDHQMVLCKKDLIRRDILYYKGRIDMDKYEVVDIEDGRDDDFNVSMKNAFKLHNKETEEIHLFFAKKLEEKIRWLRAFREERKMVQEDEKIGFEISENQKRQAAMTVRKVPKQKGVNSARSVPPSYPPPQDPLNHGQYLVPDGIAQSQVFEFTEPKRSQSPFWQNFSRLTPFKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Molecular Mass: 60.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Storage Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
• Concentration: > 0.05 μg/μL as determined by microplate BCA method
• Shipping: Dry Ice

Gene Information

• Gene Name 2: ARHGEF9 Cdc42 guanine nucleotide exchange factor (GEF) 9 [ Homo sapiens (human) ]
• Official Symbol 2: ARHGEF9
• Synonyms 2: ARHGEF9; Cdc42 guanine nucleotide exchange factor (GEF) 9; rho guanine nucleotide exchange factor 9; collybistin; KIAA0424; PEM 2; PEM-2 homolog; hPEM-2 collybistin; rac/Cdc42 guanine nucleotide exchange factor 9; PEM2; EIEE8; PEM-2; HPEM-2; COLLYBISTIN
• Gene ID 2: 23229
• mRNA Refseq 2: NM_015185
• Protein Refseq 2: NP_056000
• MIM 2: 300429
• UniProt ID 2: O43307