Recombinant Full Length Human ASIP Protein, C-Flag-tagged

• Cat.No.: ASIP-917HFL
• Product Overview: Recombinant Full Length Human ASIP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: In mice, the agouti gene encodes a paracrine signaling molecule that causes hair follicle melanocytes to synthesize pheomelanin, a yellow pigment, instead of the black or brown pigment, eumelanin. Pleiotropic effects of constitutive expression of the mouse gene include adult-onset obesity, increased tumor susceptibility, and premature infertility. This gene is highly similar to the mouse gene and encodes a secreted protein that may (1) affect the quality of hair pigmentation, (2) act as a pharmacological antagonist of alpha-melanocyte-stimulating hormone, (3) play a role in neuroendocrine aspects of melanocortin action, and (4) have a functional role in regulating lipid metabolism in adipocytes.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 12 kDa
• AA Sequence: MDVTRLLLATLLVFLCFFTANSHLP PEEKLRDDRSLRSNSSVNLLDVPSV SIVALNKKSKQIGRKAAEKK
  RSSKKEASMKKVVRPRTPLSAPCVA TRNSCKPPAPACCDPCASCQCRFFR SACSCRVLSLNCTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Secreted Protein
• Protein Pathways: Melanogenesis

Gene Information

• Gene Name: ASIP agouti signaling protein [ Homo sapiens (human) ]
• Official Symbol: ASIP
• Synonyms: ASP; AGSW; AGTI; AGTIL; SHEP9
• Gene ID: 434
• mRNA Refseq: NM_001672.3
• Protein Refseq: NP_001663.2
• MIM: 600201
• UniProt ID: P42127