Recombinant Full Length Human ATP1A1 Protein, C-Flag-tagged

• Cat.No.: ATP1A1-379HFL
• Product Overview: Recombinant Full Length Human ATP1A1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 112.7 kDa
• AA Sequence: MGKGVGRDKYEPAAVSEQGDKKGKK GKKDRDMDELKKEVSMDDHKLSLDE LHRKYGTDLSRGLTSARAAE ILARDGPNALTPPPTTPEWIKFCRQ LFGGFSMLLWIGAILCFLAYSIQAA TEEEPQNDNLYLGVVLSAVV IITGCFSYYQEAKSSKIMESFKNMV PQQALVIRNGEKMSINAEEVVVGDL VEVKGGDRIPADLRIISANG CKVDNSSLTGESEPQTRSPDFTNEN PLETRNIAFFSTNCVEGTARGIVVY TGDRTVMGRIATLASGLEGG QTPIAAEIEHFIHIITGVAVFLGVS FFILSLILEYTWLEAVIFLIGIIVA NVPEGLLATVTVCLTLTAKR MARKNCLVKNLEAVETLGSTSTICS DKTGTLTQNRMTVAHMWFDNQIHEA DTTENQSGVSFDKTSATWLA LSRIAGLCNRAVFQANQENLPILKR AVAGDASESALLKCIELCCGSVKEM RERYAKIVEIPFNSTNKYQL SIHKNPNTSEPQHLLVMKGAPERIL DRCSSILLHGKEQPLDEELKDAFQN AYLELGGLGERVLGFCHLFL PDEQFPEGFQFDTDDVNFPIDNLCF VGLISMIDPPRAAVPDAVGKCRSAG IKVIMVTGDHPITAKAIAKG VGIISEGNETVEDIAARLNIPVSQV NPRDAKACVVHGSDLKDMTSEQLDD ILKYHTEIVFARTSPQQKLI IVEGCQRQGAIVAVTGDGVNDSPAL KKADIGVAMGIAGSDVSKQAADMIL LDDNFASIVTGVEEGRLIFD NLKKSIAYTLTSNIPEITPFLIFII ANIPLPLGTVTILCIDLGTDMVPAI SLAYEQAESDIMKRQPRNPK TDKLVNERLISMAYGQIGMIQALGG FFTYFVILAENGFLPIHLLGLRVDW DDRWINDVEDSYGQQWTYEQ RKIVEFTCHTAFFVSIVVVQWADLV ICKTRRNSVFQQGMKNKILIFGLFE ETALAAFLSYCPGMGVALRM
  YPLKPTWWFCAFPYSLLIFVYDEVR KLIIRRRPGGWVEKETYYTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cardiac muscle contraction

Gene Information

• Gene Name: ATP1A1 ATPase Na+/K+ transporting subunit alpha 1 [ Homo sapiens (human) ]
• Official Symbol: ATP1A1
• Synonyms: CMT2DD; HOMGSMR2
• Gene ID: 476
• mRNA Refseq: NM_000701.8
• Protein Refseq: NP_000692.2
• MIM: 182310
• UniProt ID: P05023