Recombinant Full Length Human CAPN13 Protein, C-Flag-tagged

• Cat.No.: CAPN13-1878HFL
• Product Overview: Recombinant Full Length Human CAPN13 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes a member of the calpain large subunit family.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 76.5 kDa
• AA Sequence: MAYYQEPSVETSIIKFKDQDFTTLR DHCLSMGRTFKDETFPAADSSIGQK LLQEKRLSNVIWKRPQDLPG GPPHFILDDISRFDIQQGGAADCWF LAALGSLTQNPQYRQKILMVQSFSH QYAGIFRFRFWQCGQWVEVV IDDRLPVQGDKCLFVRPRHQNQEFW PCLLEKAYAKLLGSYSDLHYGFLED ALVDLTGGVITNIHLHSSPV DLVKAVKTATKAGSLITCATPSGPT DTAQAMENGLVSLHAYTVTGAEQIQ YRRGWEEIISLWNPWGWGET EWRGRWSDGSQEWEETCDPRKSQLH KKREDGEFWMSCQDFQQKFIAMFIC SEIPITLDHGNTLHEGWSQI MFRKQVILGNTAGGPRNDAQFNFSV QEPMEGTNVVVCVTVAVTPSNLKAE DAKFPLDFQVILAGSQRFRE KFPPVFFSSFRNTVQSSNNKFRRNF TMTYHLSPGNYVVVAQTRRKSAEFL LRIFLKMPDSDRHLSSHFNL RMKGSPSEHGSQQSIFNRYAQQRLD IDATQLQGLLNQELLTGPPGDMFSL DECRSLVALMELKVNGRLDQ EEFARLWKRLVHYQHVFQKVQTSPG VLLSSDLWKAIENTDFLRGIFISRE LLHLVTLRYSDSVGRVSFPS LVCFLMRLEAMAKTFRNLSKDGKGL YLTEMEWMSLVMYN TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protease

Gene Information

• Gene Name: CAPN13 calpain 13 [ Homo sapiens (human) ]
• Official Symbol: CAPN13
• Synonyms: FLJ23523
• Gene ID: 92291
• mRNA Refseq: NM_144575.3
• Protein Refseq: NP_653176.2
• MIM: 610228
• UniProt ID: Q6MZZ7