Recombinant Full Length Human CDC25A Protein, C-Flag-tagged

• Cat.No.: CDC25A-1987HFL
• Product Overview: Recombinant Full Length Human CDC25A Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 58.9 kDa
• AA Sequence: MELGPEPPHRRRLLFACSPPPASQP VVKALFGASAAGGLSPVTNLTVTMD QLQGLGSDYEQPLEVKNNSN LQRMGSSESTDSGFCLDSPGPLDSK ENLENPMRRIHSLPQKLLGCSPALK RSHSDSLDHDIFQLIDPDEN KENEAFEFKKPVRPVSRGCLHSHGL QEGKDLFTQRQNSAPARMLSSNERD SSEPGNFIPLFTPQSPVTAT LSDEDDGFVDLLDGENLKNEEETPS CMASLWTAPLVMRTTNLDNRCKLFD SPSLCSSSTRSVLKRPERSQ EESPPGSTKRRKSMSGASPKESTNP EKAHETLHQSLSLASSPKGTIENIL DNDPRDLIGDFSKGYLFHTV AGKHQDLKYISPEIMASVLNGKFAN LIKEFVIIDCRYPYEYEGGHIKGAV NLHMEEEVEDFLLKKPIVPT DGKRVIVVFHCEFSSERGPRMCRYV RERDRLGNEYPKLHYPELYVLKGGY KEFFMKCQSYCEPPSYRPMH HEDFKEDLKKFRTKSRTWAGEKSKR EMYSRLKKL TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle, Progesterone-mediated oocyte maturation

Gene Information

• Gene Name: CDC25A cell division cycle 25A [ Homo sapiens (human) ]
• Official Symbol: CDC25A
• Synonyms: CDC25A2
• Gene ID: 993
• mRNA Refseq: NM_001789.3
• Protein Refseq: NP_001780.2
• MIM: 116947
• UniProt ID: P30304