Recombinant Full Length Human cereblon and DDB1 Complex Protein, His tagged

• Cat.No.: CRBN-DDB1-21HFL
• Product Overview: Recombinant full-length human CRBN and DDB1 were expressed by baculovirus in Sf9 insect cells using N-terminal His-tag for both proteins. The CRBN and DDB1 gene accession numbers are NM_016302 and NM_001923, respectively.

Specification

• Species: Human
• Source: Insect cells
• Tag: His
• Protein Length: CRBN: 1-442aa
  DDB1: 1-1140aa
• Description: CRBN (Cereblon) and DDB1 (DNA damage-binding protein 1) are proteins that play crucial roles in various cellular processes, including DNA repair, cell cycle regulation, and protein degradation. CRBN is a substrate receptor protein, while DDB1 acts as an adaptor protein within the Cullin-RING ubiquitin ligase (CRL) E3 ligase complex. This complex plays a critical role in the ubiquitin proteasome pathway, which controls the targeted degradation of specific proteins. Understanding the intricate roles of CRBN and DDB1 in cellular processes is crucial for deciphering their potential as therapeutic targets and advancing our knowledge of various diseases, particularly cancer, where dysregulation of these proteins has been observed.
• Tag: N-His
• Molecular Mass: CRBN: ~51 kDa
  DDB1: ~131 kDa
• Purity: > 80%
• Stability: 1yr at –70 centigrade from date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Storage Buffer: Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.25mM DTT, 25% glycerol.
• Concentration: > 0.05 μg/μL
• Shipping: Dry ice
• GeneID 2: 1642

Gene Information

• Gene Name: CRBN cereblon [ Homo sapiens (human) ]
• Official Symbol: CRBN
• Synonyms: CRBN; cereblon; mental retardation, non syndromic, autosomal recessive, 2A , MRT2A; protein cereblon; protein x 0001; MRT2; MRT2A; MGC27358; DKFZp781K0715
• Gene ID: 51185
• mRNA Refseq: NM_016302
• Protein Refseq: NP_057386
• MIM: 609262
• UniProt ID: Q96SW2
• Gene Name 2: DDB1 damage-specific DNA binding protein 1, 127kDa [ Homo sapiens (human) ]
• Official Symbol 2: DDB1
• Synonyms 2: DDB1; damage-specific DNA binding protein 1, 127kDa; damage specific DNA binding protein 1 (127kD); DNA damage-binding protein 1; XPE; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; damage-specific DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
• mRNA Refseq 2: NM_001923
• Protein Refseq 2: NP_001914
• MIM 2: 600045
• UniProt ID 2: Q16531