Recombinant Full Length Human CH25H Protein, C-Flag-tagged

• Cat.No.: CH25H-1709HFL
• Product Overview: Recombinant Full Length Human CH25H Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 31.6 kDa
• AA Sequence: MSCHNCSDPQVLCSSGQLFLQPLWD HLRSWEALLQSPFFPVIFSITTYVG FCLPFVVLDILCSWVPALRR YKIHPDFSPSAQQLLPCLGQTLYQH VMFVFPVTLLHWARSPALLPHEAPE LLLLLHHILFCLLLFDMEFF VWHLLHHKVPWLYRTFHKVHHQNSS SFALATQYMSVWELFSLGFFDMMNV TLLGCHPLTTLTFHVVNIWL
  SVEDHSGYNFPWSTHRLVPFGWYGG VVHHDLHHSHFNCNFAPYFTHWDKI LGTLRTASVPARTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Primary bile acid biosynthesis

Gene Information

• Gene Name: CH25H cholesterol 25-hydroxylase [ Homo sapiens (human) ]
• Official Symbol: CH25H
• Synonyms: C25H
• Gene ID: 9023
• mRNA Refseq: NM_003956.4
• Protein Refseq: NP_003947.1
• MIM: 604551
• UniProt ID: O95992