Recombinant Human CH25H Protein, His-tagged

• Cat.No.: CH25H-771H
• Product Overview: Recombinant Human CH25H Protein (Trp142-Asn247) with His tag was expressed in E. coli.

Specification

• Description: This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases, this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates.
• Source: E. coli
• Species: Human
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 16.4 kDa
• Protein length: Trp142-Asn247
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: CH25H cholesterol 25-hydroxylase [ Homo sapiens (human) ]
• Official Symbol: CH25H
• Synonyms: CH25H; cholesterol 25-hydroxylase; h25OH; cholesterol 25-monooxygenase; C25H
• Gene ID: 9023
• mRNA Refseq: NM_003956
• Protein Refseq: NP_003947
• MIM: 604551
• UniProt ID: O95992