Recombinant Full Length Human CHAF1B Protein, C-Flag-tagged

• Cat.No.: CHAF1B-51HFL
• Product Overview: Recombinant Full Length Human CHAF1B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 61.3 kDa
• AA Sequence: MKVITCEIAWHNKEPVYSLDFQHGT AGRIHRLASAGVDTNVRIWKVEKGP DGKAIVEFLSNLARHTKAVN VVRFSPTGEILASGGDDAVILLWKV NDNKEPEQIAFQDEDEAQLNKENWT VVKTLRGHLEDVYDICWATD GNLMASASVDNTAIIWDVSKGQKIS IFNEHKSYVQGVTWDPLGQYVATLS CDRVLRVYSIQKKRVAFNVS KMLSGIGAEGEARSYRMFHDDSMKS FFRRLSFTPDGSLLLTPAGCVESGE NVMNTTYVFSRKNLKRPIAH LPCPGKATLAVRCCPVYFELRPVVE TGVELMSLPYRLVFAVASEDSVLLY DTQQSFPFGYVSNIHYHTLS DISWSSDGAFLAISSTDGYCSFVTF EKDELGIPLKEKPVLNMRTPDTAKK TKSQTHRGSSPGPRPVEGTP ASRTQDPSSPGTTPPQARQAPAPTV IRDPPSITPAVKSPLPGPSEEKTLQ PSSQNTKAHPSRRVTLNTLQ AWSKTTPRRINLTPLKTDTPPSSVP TSVISTPSTEEIQSETPGDAQGSPP ELKRPRLDENKGGTESLDPTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: CHAF1B chromatin assembly factor 1 subunit B [ Homo sapiens (human) ]
• Official Symbol: CHAF1B
• Synonyms: CAF1; MPP7; CAF-1; CAF1A; CAF1P60; CAF-IP60; MPHOSPH7
• Gene ID: 8208
• mRNA Refseq: NM_005441.3
• Protein Refseq: NP_005432.1
• MIM: 601245
• UniProt ID: Q13112