Recombinant Full Length Human CYP2D6 Protein, C-Flag-tagged

• Cat.No.: CYP2D6-1918HFL
• Product Overview: Recombinant Full Length Human CYP2D6 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 49.8 kDa
• AA Sequence: MGLEALVPLAMIVAIFLLLVDLMHR RQRWAARYPPGPLPLPGLGNLLHVD FQNTPYCFDQLRRRFGDVFS LQLAWTPVVVLNGLAAVREALVTHG EDTADRPPVPITQILGFGPRSQGRP FRPNGLLDKAVSNVIASLTC GRRFEYDDPRFLRLLDLAQEGLKEE SGFLREVLNAVPVLLHIPALAGKVL RFQKAFLTQLDELLTEHRMT WDPAQPPRDLTEAFLAEMEKAKGNP ESSFNDENLCIVVADLFSAGMVTTS TTLAWGLLLMILHPDVQRRV QQEIDDVIGQVRRPEMGDQAHMPYT TAVIHEVQRFGDIVPLGVTHMTSRD IEVQGFRIPKGTTLITNLSS VLKDEAVWEKPFRFHPEHFLDAQGH FVKPEAFLPFSAGRRACLGEPLARM ELFLFFTSLLQHFSFSVPTG QPRPSHHGVFAFLVTPSPYELCAVP R TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Drug metabolism - cytochrome P450

Gene Information

• Gene Name: CYP2D6 cytochrome P450 family 2 subfamily D member 6 [ Homo sapiens (human) ]
• Official Symbol: CYP2D6
• Synonyms: CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
• Gene ID: 1565
• mRNA Refseq: NM_001025161.3
• Protein Refseq: NP_001020332.2
• MIM: 124030
• UniProt ID: P10635