Recombinant Full Length Human D2HGDH Protein, C-Flag-tagged

• Cat.No.: D2HGDH-1752HFL
• Product Overview: Recombinant Full Length Human D2HGDH Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes D-2hydroxyglutarate dehydrogenase, a mitochondrial enzyme belonging to the FAD-binding oxidoreductase/transferase type 4 family. This enzyme, which is most active in liver and kidney but also active in heart and brain, converts D-2-hydroxyglutarate to 2-ketoglutarate. Mutations in this gene are present in D-2-hydroxyglutaric aciduria, a rare recessive neurometabolic disorder causing developmental delay, epilepsy, hypotonia, and dysmorphic features.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.8 kDa
• AA Sequence: MLPRRPLAWPAWLLRGAPGAAGSWG RPVGPLARRGCCSAPGTPEVPLTRE RYPVQRLPFSTVSKQDLAAF ERIVPGGVVTDPEALQAPNVDWLRT LRGCSKVLLRPRTSEEVSHILRHCH ERNLAVNPQGGNTGMVGGSV PVFDEIILSTARMNRVLSFHSVSGI LVCQAGCVLEELSRYVEERDFIMPL DLGAKGSCHIGGNVATNAGG LRFLRYGSLHGTVLGLEVVLADGTV LDCLTSLRKDNTGYDLKQLFIGSEG TLGIITTVSILCPPKPRAVN VAFLGCPGFAEVLQTFSTCKGMLGE ILSAFEFMDAVCMQLVGRHLHLASP VQESPFYVLIETSGSNAGHD AEKLGHFLEHALGSGLVTDGTMATD QRKVKMLWALRERITEALSRDGYVY KYDLSLPVERLYDIVTDLRA RLGPHAKHVVGYGHLGDGNLHLNVT AEAFSPSLLAALEPHVYEWTAGQQG SVSAEHGVGFRKRDVLGYSK
  PPGALQLMQQLKALLDPKGILNPYK TLPSQATRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: D2HGDH D-2-hydroxyglutarate dehydrogenase [ Homo sapiens (human) ]
• Official Symbol: D2HGDH
• Synonyms: D2HGD
• Gene ID: 728294
• mRNA Refseq: NM_152783.5
• Protein Refseq: NP_689996.4
• MIM: 609186
• UniProt ID: Q8N465