Recombinant Full Length Human DARS1 Protein, C-Flag-tagged

• Cat.No.: DARS1-1036HFL
• Product Overview: Recombinant Full Length Human DARS1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs. The encoded protein ligates L-aspartate to tRNA(Asp). Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 57 kDa
• AA Sequence: MPSASASRKSQEKPREIMDAAEDYA KERYGISSMIQSQEKPDRVLVRVRD LTIQKADEVVWVRARVHTSR AKGKQCFLVLRQQQFNVQALVAVGD HASKQMVKFAANINKESIVDVEGVV RKVNQKIGSCTQQDVELHVQ KIYVISLAEPRLPLQLDDAVRPEAE GEEEGRATVNQDTRLDNRVIDLRTS TSQAVFRLQSGICHLFRETL INKGFVEIQTPKIISAASEGGANVF TVSYFKNNAYLAQSPQLYKQMCICA DFEKVFSIGPVFRAEDSNTH RHLTEFVGLDIEMAFNYHYHEVMEE IADTMVQIFKGLQERFQTEIQTVNK QFPCEPFKFLEPTLRLEYCE ALAMLREAGVEMGDEDDLSTPNEKL LGHLVKEKYDTDFYILDKYPLAVRP FYTMPDPRNPKQSNSYDMFM RGEEILSGAQRIHDPQLLTERALHH GIDLEKIKAYIDSFRFGAPPHAGGG IGLERVTMLFLGLHNVRQTS
  MFPRDPKRLTPTRTRPLEQKLISEE DLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Aminoacyl-tRNA biosynthesis

Gene Information

• Gene Name: DARS1 aspartyl-tRNA synthetase 1 [ Homo sapiens (human) ]
• Official Symbol: DARS1
• Synonyms: DARS; HBSL; aspRS
• Gene ID: 1615
• mRNA Refseq: NM_001349.4
• Protein Refseq: NP_001340.2
• MIM: 603084
• UniProt ID: P14868