Recombinant Full Length Human DNAJB1 Protein, C-Flag-tagged

• Cat.No.: DNAJB1-1382HFL
• Product Overview: Recombinant Full Length Human DNAJB1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.9 kDa
• AA Sequence: MGKDYYQTLGLARGASDEEIKRAYR RQALRYHPDKNKEPGAEEKFKEIAE AYDVLSDPRKREIFDRYGEE GLKGSGPSGGSGGGANGTSFSYTFH GDPHAMFAEFFGGRNPFDTFFGQRN GEEGMDIDDPFSGFPMGMGG FTNVNFGRSRSAQEPARKKQDPPVT HDLRVSLEEIYSGCTKKMKISHKRL NPDGKSIRNEDKILTIEVKK GWKEGTKITFPKEGDQTSNNIPADI VFVLKDKPHNIFKRDGSDVIYPARI SLREALCGCTVNVPTLDGRT
  IPVVFKDVIRPGMRRKVPGEGLPLP KTPEKRGDLIIEFEVIFPERIPQTS RTVLEQVLPI
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.

Gene Information

• Gene Name: DNAJB1 DnaJ heat shock protein family (Hsp40) member B1 [ Homo sapiens (human) ]
• Official Symbol: DNAJB1
• Synonyms: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Gene ID: 3337
• mRNA Refseq: NM_006145.3
• Protein Refseq: NP_006136.1
• MIM: 604572
• UniProt ID: P25685