Recombinant Full Length Human DTL Protein, C-Flag-tagged

• Cat.No.: DTL-1751HFL
• Product Overview: Recombinant Full Length Human DTL Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Contributes to ubiquitin-protein transferase activity. Involved in several processes, including protein ubiquitination; regulation of G2/M transition of mitotic cell cycle; and translesion synthesis. Located in centrosome; cytosol; and nuclear lumen. Part of Cul4A-RING E3 ubiquitin ligase complex and Cul4B-RING E3 ubiquitin ligase complex.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 79.3 kDa
• AA Sequence: MLFNSVLRQPQLGVLRNGWSSQYPL QSLLTGYQCSGNDEHTSYGETGVPV PPFGCTFSSAPNMEHVLAVA NEEGFVRLYNTESQSFRKKCFKEWM AHWNAVFDLAWVPGELKLVTAAGDQ TAKFWDVKAGELIGTCKGHQ CSLKSVAFSKFEKAVFCTGGRDGNI MVWDTRCNKKDGFYRQVNQISGAHN TSDKQTPSKPKKKQNSKGLA PSVDFQQSVTVVLFQDENTLVSAGA VDGIIKVWDLRKNYTAYRQEPIASK SFLYPGSSTRKLGYSSLILD STGSTLFANCTDDNIYMFNMTGLKT SPVAIFNGHQNSTFYVKSSLSPDDQ FLVSGSSDEAAYIWKVSTPW QPPTVLLGHSQEVTSVCWCPSDFTK IATCSDDNTLKIWRLNRGLEEKPGG DKLSTVGWASQKKKESRPGL VTVTSSQSTPAKAPRVKCNPSNSSP SSAACAPSCAGDLPLPSNTPTFSIK TSPAKARSPINRRGSVSSVS PKPPSSFKMSIRNWVTRTPSSSPPI TPPASETKIMSPRKALIPVSQKSSQ AEACSESRNRVKRRLDSSCL ESVKQKCVKSCNCVTELDGQVENLH LDLCCLAGNQEDLSKDSLGPTKSSK IEGAGTSISEPPSPISPYAS ESCGTLPLPLRPCGEGSEMVGKENS SPENKNWLLAMAAKRKAENPSPRSP SSQTPNSRRQSGKTLPSPVT
  ITPSSMRKICTYFHRKSQEDFCGPE HSTELTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome

Gene Information

• Gene Name: DTL denticleless E3 ubiquitin protein ligase homolog [ Homo sapiens (human) ]
• Official Symbol: DTL
• Synonyms: CDT2; RAMP; DCAF2; L2DTL
• Gene ID: 51514
• mRNA Refseq: NM_016448.4
• Protein Refseq: NP_057532.4
• MIM: 610617
• UniProt ID: Q9NZJ0