Recombinant Full Length Human EBP Protein, C-Flag-tagged

• Cat.No.: EBP-579HFL
• Product Overview: Recombinant Full Length Human EBP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome).
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 26.2 kDa
• AA Sequence: MTTNAGPLHPYWPQHLRLDNFVPND RPTWHILAGLFSVTGVLVVTTWLLS GRAAVVPLGTWRRLSLCWFA VCGFIHLVIEGWFVLYYEDLLGDQA FLSQLWKEYAKGDSRYILGDNFTVC METITACLWGPLSLWVVIAF LRQHPLRFILQLVVSVGQIYGDVLY FLTEHRDGFQHGELGHPLYFWFYFV FMNALWLVLPGVLVLDAVKH
  LTHAQSTLDAKATKAKSKKNTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis

Gene Information

• Gene Name: EBP EBP cholestenol delta-isomerase [ Homo sapiens (human) ]
• Official Symbol: EBP
• Synonyms: CPX; CHO2; CPXD; MEND; CDPX2
• Gene ID: 10682
• mRNA Refseq: NM_006579.3
• Protein Refseq: NP_006570.1
• MIM: 300205
• UniProt ID: Q15125