Recombinant Full Length Human EPHB4 Protein, C-Flag-tagged

• Cat.No.: EPHB4-1774HFL
• Product Overview: Recombinant Full Length Human EPHB4 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 106.6 kDa
• AA Sequence: MELRVLLCWASLAAALEETLLNTKL ETADLKWVTFPQVDGQWEELSGLDE EQHSVRTYEVCDVQRAPGQA HWLRTGWVPRRGAVHVYATLRFTML ECLSLPRAGRSCKETFTVFYYESDA DTATALTPAWMENPYIKVDT VAAEHLTRKRPGAEATGKVNVKTLR LGPLSKAGFYLAFQDQGACMALLSL HLFYKKCAQLTVNLTRFPET VPRELVVPVAGSCVVDAVPAPGPSP SLYCREDGQWAEQPVTGCSCAPGFE AAEGNTKCRACAQGTFKPLS GEGSCQPCPANSHSNTIGSAVCQCR VGYFRARTDPRGAPCTTPPSAPRSV VSRLNGSSLHLEWSAPLESG GREDLTYALRCRECRPGGSCAPCGG DLTFDPGPRDLVEPWVVVRGLRPDF TYTFEVTALNGVSSLATGPV PFEPVNVTTDREVPPAVSDIRVTRS SPSSLSLAWAVPRAPSGAVLDYEVK YHEKGAEGPSSVRFLKTSEN RAELRGLKRGASYLVQVRARSEAGY GPFGQEHHSQTQLDESEGWREQLAL IAGTAVVGVVLVLVVIVVAV LCLRKQSNGREAEYSDKHGQYLIGH GTKVYIDPFTYEDPNEAVREFAKEI DVSYVKIEEVIGAGEFGEVC RGRLKAPGKKESCVAIKTLKGGYTE RQRREFLSEASIMGQFEHPNIIRLE GVVTNSMPVMILTEFMENGA LDSFLRLNDGQFTVIQLVGMLRGIA SGMRYLAEMSYVHRDLAARNILVNS NLVCKVSDFGLSRFLEENSS DPTYTSSLGGKIPIRWTAPEAIAFR KFTSASDAWSYGIVMWEVMSFGERP YWDMSNQDVINAIEQDYRLP PPPDCPTSLHQLMLDCWQKDRNARP RFPQVVSALDKMIRNPASLKIVARE NGGASHPLLDQRQPHYSAFG SVGEWLRAIKMGRYEESFAAAGFGS FELVSQISAEDLLRIGVTLAGHQKK ILASVQHMKSQAKPGTPGGT GGPAPQY TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Axon guidance

Gene Information

• Gene Name: EPHB4 EPH receptor B4 [ Homo sapiens (human) ]
• Official Symbol: EPHB4
• Synonyms: HTK; MYK1; HFASD; CMAVM2; LMPHM7; TYRO11
• Gene ID: 2050
• mRNA Refseq: NM_004444.5
• Protein Refseq: NP_004435.3
• MIM: 600011
• UniProt ID: P54760