Recombinant Full Length Human ERBB3 Protein, C-Flag-tagged

• Cat.No.: ERBB3-411HFL
• Product Overview: Recombinant Full Length Human ERBB3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 146 kDa
• AA Sequence: MRANDALQVLGLLFSLARGSEVGNS QAVCPGTLNGLSVTGDAENQYQTLY KLYERCEVVMGNLEIVLTGH NADLSFLQWIREVTGYVLVAMNEFS TLPLPNLRVVRGTQVYDGKFAIFVM LNYNTNSSHALRQLRLTQLT EILSGGVYIEKNDKLCHMDTIDWRD IVRDRDAEIVVKDNGRSCPPCHEVC KGRCWGPGSEDCQTLTKTIC APQCNGHCFGPNPNQCCHDECAGGC SGPQDTDCFACRHFNDSGACVPRCP QPLVYNKLTFQLEPNPHTKY QYGGVCVASCPHNFVVDQTSCVRAC PPDKMEVDKNGLKMCEPCGGLCPKA CEGTGSGSRFQTVDSSNIDG FVNCTKILGNLDFLITGLNGDPWHK IPALDPEKLNVFRTVREITGYLNIQ SWPPHMHNFSVFSNLTTIGG RSLYNRGFSLLIMKNLNVTSLGFRS LKEISAGRIYISANRQLCYHHSLNW TKVLRGPTEERLDIKHNRPR RDCVAEGKVCDPLCSSGGCWGPGPG QCLSCRNYSRGGVCVTHCNFLNGEP REFAHEAECFSCHPECQPME GTATCNGSGSDTCAQCAHFRDGPHC VSSCPHGVLGAKGPIYKYPDVQNEC RPCHENCTQGCKGPELQDCL GQTLVLIGKTHLTMALTVIAGLVVI FMMLGGTFLYWRGRRIQNKRAMRRY LERGESIEPLDPSEKANKVL ARIFKETELRKLKVLGSGVFGTVHK GVWIPEGESIKIPVCIKVIEDKSGR QSFQAVTDHMLAIGSLDHAH IVRLLGLCPGSSLQLVTQYLPLGSL LDHVRQHRGALGPQLLLNWGVQIAK GMYYLEEHGMVHRNLAARNV LLKSPSQVQVADFGVADLLPPDDKQ LLYSEAKTPIKWMALESIHFGKYTH QSDVWSYGVTVWELMTFGAE PYAGLRLAEVPDLLEKGERLAQPQI CTIDVYMVMVKCWMIDENIRPTFKE LANEFTRMARDPPRYLVIKR ESGPGIAPGPEPHGLTNKKLEEVEL EPELDLDLDLEAEEDNLATTTLGSA LSLPVGTLNRPRGSQSLLSP SSGYMPMNQGNLGESCQESAVSGSS ERCPRPVSLHPMPRGCLASESSEGH VTGSEAELQEKVSMCRSRSR SRSPRPRGDSAYHSQRHSLLTPVTP LSPPGLEEEDVNGYVMPDTHLKGTP SSREGTLSSVGLSSVLGTEE EDEDEEYEYMNRRRRHSPPHPPRPS SLEELGYEYMDVGSDLSASLGSTQS CPLHPVPIMPTAGTTPDEDY EYMNRQRDGGGPGGDYAAMGACPAS EQGYEEMRAFQGPGHQAPHVHYARL KTLRSLEATDSAFDNPDYWH
  SRLFPKANAQRTTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Adult stem cells, Druggable Genome, Protein Kinase, Secreted Protein, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway

Gene Information

• Gene Name: ERBB3 erb-b2 receptor tyrosine kinase 3 [ Homo sapiens (human) ] Gene ID: 2065, updated on 30-Oct-2022 Download Datasets
• Official Symbol: ERBB3
• Synonyms: HER3; FERLK; LCCS2; VSCN1; ErbB-3; c-erbB3; erbB3-S; MDA-BF-1; c-erbB-3; p180-ErbB3; p45-sErbB3; p85-sErbB3
• Gene ID: 2065
• mRNA Refseq: NM_001982.4
• Protein Refseq: NP_001973.2
• MIM: 190151
• UniProt ID: P21860