Recombinant Full Length Human GLUD1 Protein, C-Flag-tagged

• Cat.No.: GLUD1-1278HFL
• Product Overview: Recombinant Full Length Human GLUD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 56 kDa
• AA Sequence: MYRYLGEALLLSRAGPAALGSASAD SAALLGWARGQPAAAPQPGLALAAR RHYSEAVADREDDPNFFKMV EGFFDRGASIVEDKLVEDLRTRESE EQKRNRVRGILRIIKPCNHVLSLSF PIRRDDGSWEVIEGYRAQHS QHRTPCKGGIRYSTDVSVDEVKALA SLMTYKCAVVDVPFGGAKAGVKINP KNYTDNELEKITRRFTMELA KKGFIGPGIDVPAPDMSTGEREMSW IADTYASTIGHYDINAHACVTGKPI SQGGIHGRISATGRGVFHGI ENFINEASYMSILGMTPGFGDKTFV VQGFGNVGLHSMRYLHRFGAKCIAV GESDGSIWNPDGIDPKELED FKLQHGSILGFPKAKPYEGSILEAD CDILIPAASEKQLTKSNAPRVKAKI IAEGANGPTTPEADKIFLER NIMVIPDLYLNAGGVTVSYFEWLKN LNHVSYGRLTFKYERDSNYHLLMSV QESLERKFGKHGGTIPIVPT AEFQDRISGASEKDIVHSGLAYTME RSARQIMRTAMKYNLGLDLRTAAYV NAIEKVFKVYNEAGVTFTTRTRPLE QKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism

Gene Information

• Gene Name: GLUD1 glutamate dehydrogenase 1 [ Homo sapiens (human) ]
• Official Symbol: GLUD1
• Synonyms: GDH; GDH1; GLUD; hGDH1
• Gene ID: 2746
• mRNA Refseq: NM_005271.5
• Protein Refseq: NP_005262.1
• MIM: 138130
• UniProt ID: P00367