Recombinant Full Length Human GPLD1 Protein, C-Flag-tagged

• Cat.No.: GPLD1-419HFL
• Product Overview: Recombinant Full Length Human GPLD1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 89.8 kDa
• AA Sequence: MSAFRLWPGLLIMLGSLCHRGSPCG LSTHVEIGHRALEFLQLHNGRVNYR ELLLEHQDAYQAGIVFPDCF YPSICKGGKFHDVSESTHWTPFLNA SVHYIRENYPLPWEKDTEKLVAFLF GITSHMAADVSWHSLGLEQG FLRTMGAIDFHGSYSEAHSAGDFGG DVLSQFEFNFNYLARRWYVPVKDLL GIYEKLYGRKVITENVIVDC SHIQFLEMYGEMLAVSKLYPTYSTK SPFLVEQFQEYFLGGLDDMAFWSTN IYHLTSFMLENGTSDCNLPE NPLFIACGGQQNHTQGSKMQKNDFH RNLTTSLTESVDRNINYTERGVFFS VNSWTPDSMSFIYKALERNI RTMFIGGSQLSQKHVSSPLASYFLS FPYARLGWAMTSADLNQDGHGDLVV GAPGYSRPGHIHIGRVYLIY GNDLGLPPVDLDLDKEAHRILEGFQ PSGRFGSALAVLDFNVDGVPDLAVG APSVGSEQLTYKGAVYVYFG SKQGGMSSSPNITISCQDIYCNLGW TLLAADVNGDSEPDLVIGSPFAPGG GKQKGIVAAFYSGPSLSDKE KLNVEAANWTVRGEEDFSWFGYSLH GVTVDNRTLLLVGSPTWKNASRLGH LLHIRDEKKSLGRVYGYFPP NGQSWFTISGDKAMGKLGTSLSSGH VLMNGTLKQVLLVGAPTYDDVSKVA FLTVTLHQGGATRMYALISD AQPLLLSTFSGDRRFSRFGGVLHLS DLDDDGLDEIIMAAPLRIADVTSGL IGGEDGRVYVYNGKETTLGD MTGKCKSWITPCPEEKAQYVLISPE ASSRFGSSLITVRSKAKNQVVIAAG RSSLGARLSGALHVYSLGSDTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Glycosylphosphatidylinositol(GPI)-anchor biosynthesis

Gene Information

• Gene Name: GPLD1 glycosylphosphatidylinositol specific phospholipase D1 [ Homo sapiens (human) ]
• Official Symbol: GPLD1
• Synonyms: PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1
• Gene ID: 2822
• mRNA Refseq: NM_001503.4
• Protein Refseq: NP_001494.2
• MIM: 602515
• UniProt ID: P80108