Recombinant Full Length Human GRK2 Protein, C-Flag-tagged

• Cat.No.: GRK2-1271HFL
• Product Overview: Recombinant Full Length Human GRK2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 79.4 kDa
• AA Sequence: MADLEAVLADVSYLMAMEKSKATPA ARASKKILLPEPSIRSVMQKYLEDR GEVTFEKIFSQKLGYLLFRD FCLNHLEEARPLVEFYEEIKKYEKL ETEEERVARSREIFDSYIMKELLAC SHPFSKSATEHVQGHLGKKQ VPPDLFQPYIEEICQNLRGDVFQKF IESDKFTRFCQWKNVELNIHLTMND FSVHRIIGRGGFGEVYGCRK ADTGKMYAMKCLDKKRIKMKQGETL ALNERIMLSLVSTGDCPFIVCMSYA FHTPDKLSFILDLMNGGDLH YHLSQHGVFSEADMRFYAAEIILGL EHMHNRFVVYRDLKPANILLDEHGH VRISDLGLACDFSKKKPHAS VGTHGYMAPEVLQKGVAYDSSADWF SLGCMLFKLLRGHSPFRQHKTKDKH EIDRMTLTMAVELPDSFSPE LRSLLEGLLQRDVNRRLGCLGRGAQ EVKESPFFRSLDWQMVFLQKYPPPL IPPRGEVNAADAFDIGSFDE EDTKGIKLLDSDQELYRNFPLTISE RWQQEVAETVFDTINAETDRLEARK KAKNKQLGHEEDYALGKDCI MHGYMSKMGNPFLTQWQRRYFYLFP NRLEWRGEGEAPQSLLTMEEIQSVE ETQIKERKCLLLKIRGGKQF
  ILQCDSDPELVQWKKELRDAYREAQ QLVQRVPKMKNKPRSPVVELSKVPL VQRGSANGLSGPSGPTRTRRLEQKL ISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis

Gene Information

• Gene Name: GRK2 G protein-coupled receptor kinase 2 [ Homo sapiens (human) ]
• Official Symbol: GRK2
• Synonyms: BARK1; ADRBK1; BETA-ARK1
• Gene ID: 156
• mRNA Refseq: NM_001619.5
• Protein Refseq: NP_001610.2
• MIM: 109635
• UniProt ID: P25098