Recombinant Full Length Human GUCY2D Protein, C-Flag-tagged

• Cat.No.: GUCY2D-1540HFL
• Product Overview: Recombinant Full Length Human GUCY2D Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a retina-specific guanylate cyclase, which is a member of the membrane guanylyl cyclase family. Like other membrane guanylyl cyclases, this enzyme has a hydrophobic amino-terminal signal sequence followed by a large extracellular domain, a single membrane spanning domain, a kinase homology domain, and a guanylyl cyclase catalytic domain. In contrast to other membrane guanylyl cyclases, this enzyme is not activated by natriuretic peptides. Mutations in this gene result in Leber congenital amaurosis and cone-rod dystrophy-6 diseases.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 119.9 kDa
• AA Sequence: MTACARRAGGLPDPGLCGPAWWAPS LPRLPRALPRLPLLLLLLLLQPPAL SAVFTVGVLGPWACDPIFSR ARPDLAARLAAARLNRDPGLAGGPR FEVALLPEPCRTPGSLGAVSSALAR VSGLVGPVNPAACRPAELLA EEAGIALVPWGCPWTQAEGTTAPAV TPAADALYALLRAFGWARVALVTAP QDLWVEAGRSLSTALRARGL PVASVTSMEPLDLSGAREALRKVRD GPRVTAVIMVMHSVLLGGEEQRYLL EAAEELGLTDGSLVFLPFDT IHYALSPGPEALAALANSSQLRRAH DAVLTLTRHCPSEGSVLDSLRRAQE RRELPSDLNLQQVSPLFGTI YDAVFLLARGVAEARAAAGGRWVSG AAVARHIRDAQVPGFCGDLGGDEEP PFVLLDTDAAGDRLFATYML DPARGSFLSAGTRMHFPRGGSAPGP DPSCWFDPNNICGGGLEPGLVFLGF LLVVGMGLAGAFLAHYVRHR LLHMQMVSGPNKIILTVDDITFLHP HGGTSRKVAQGSRSSLGARSMSDIR SGPSQHLDSPNIGVYEGDRV WLKKFPGDQHIAIRPATKTAFSKLQ ELRHENVALYLGLFLARGAEGPAAL WEGNLAVVSEHCTRGSLQDL LAQREIKLDWMFKSSLLLDLIKGIR YLHHRGVAHGRLKSRNCIVDGRFVL KITDHGHGRLLEAQKVLPEP PRAEDQLWTAPELLRDPALERRGTL AGDVFSLAIIMQEVVCRSAPYAMLE LTPEEVVQRVRSPPPLCRPL VSMDQAPVECILLMKQCWAEQPELR PSMDHTFDLFKNINKGRKTNIIDSM LRMLEQYSSNLEDLIRERTE ELELEKQKTDRLLTQMLPPSVAEAL KTGTPVEPEYFEQVTLYFSDIVGFT TISAMSEPIEVVDLLNDLYT LFDAIIGSHDVYKVETIGDAYMVAS GLPQRNGQRHAAEIANMSLDILSAV GTFRMRHMPEVPVRIRIGLH SGPCVAGVVGLTMPRYCLFGDTVNT ASRMESTGLPYRIHVNLSTVGILRA LDSGYQVELRGRTELKGKGA
  EDTFWLVGRRGFNKPIPKPPDLQPG SSNHGISLQEIPPERRRKLEKARPG QFSTRTRPLEQKLISEEDLAANDIL DYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Olfactory transduction, Purine metabolism

Gene Information

• Gene Name: GUCY2D guanylate cyclase 2D, retinal [ Homo sapiens (human) ]
• Official Symbol: GUCY2D
• Synonyms: LCA; CG-E; CYGD; LCA1; RCD2; CACD1; CORD5; CORD6; GUC2D; ROSGC; retGC; CSNB1I; GUC1A4; RETGC-1; ROS-GC1
• Gene ID: 3000
• mRNA Refseq: NM_000180.4
• Protein Refseq: NP_000171.1
• MIM: 600179
• UniProt ID: Q02846