Recombinant Full Length Human HAAO Protein, C-Flag-tagged

• Cat.No.: HAAO-643HFL
• Product Overview: Recombinant Full Length Human HAAO Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: 3-Hydroxyanthranilate 3,4-dioxygenase is a monomeric cytosolic protein belonging to the family of intramolecular dioxygenases containing nonheme ferrous iron. It is widely distributed in peripheral organs, such as liver and kidney, and is also present in low amounts in the central nervous system. HAAO catalyzes the synthesis of quinolinic acid (QUIN) from 3-hydroxyanthranilic acid. QUIN is an excitotoxin whose toxicity is mediated by its ability to activate glutamate N-methyl-D-aspartate receptors. Increased cerebral levels of QUIN may participate in the pathogenesis of neurologic and inflammatory disorders. HAAO has been suggested to play a role in disorders associated with altered tissue levels of QUIN.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 32.4 kDa
• AA Sequence: MERRLGVRAWVKENRGSFQPPVCNK LMHQEQLKVMFIGGPNTRKDYHIEE GEEVFYQLEGDMVLRVLEQG KHRDVVIRQGEIFLLPARVPHSPQR FANTVGLVVERRRLETELDGLRYYV GDTMDVLFEKWFYCKDLGTQ LAPIIQEFFSSEQYRTGKPIPDQLL KEPPFPLSTRSIMEPMSLDAWLDSH HRELQAGTPLSLFGDTYETQ VIAYGQGSSEGLRQNVDVWLWQLEG SSVVTMGGRRLSLAPDDSLLVLAGT SYAWERTQGSVALSVTQDPA
  CKKPLGTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Metabolic pathways, Tryptophan metabolism

Gene Information

• Gene Name: HAAO 3-hydroxyanthranilate 3,4-dioxygenase [ Homo sapiens (human) ]
• Official Symbol: HAAO
• Synonyms: HAO; 3-HAO; VCRL1; h3HAO
• Gene ID: 23498
• mRNA Refseq: NM_012205.3
• Protein Refseq: NP_036337.2
• MIM: 604521
• UniProt ID: P46952