Recombinant Full Length Human HIF1A Protein, N-GST-tagged

• Cat.No.: HIF1A-01H
• Product Overview: Human HIF1A full-length ORF ( NP_851397.1, 1 a.a. - 735 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Form: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
• Molecular Mass: 109.1 kDa
• Protein Length: 1-735aa
• AA Sequence: MEGAGGANDKKKISSERRKEKSRDA ARSRRSKESEVFYELAHQLPLPHNV SSHLDKASVMRLTISYLRVRKLLDA GDLDIEDDMKAQMNCFYLKALDGFV MVLTDDGDMIYISDNVNKYMGLTQF ELTGHSVFDFTHPCDHEEMREMLTH RNGLVKKGKEQNTQRSFFLRMKCTL TSRGRTMNIKSATWKVLHCTGHIHV YDTNSNQPQCGYKKPPMTCLVLICE PIPHPSNIEIPLDSKTFLSRHSLDM KFSYCDERITELMGYEPEELLGRSI YEYYHALDSDHLTKTHHDMFTKGQV TTGQYRMLAKRGGYVWVETQATVIY NTKNSQPQCIVCVNYVVSGIIQHDL IFSLQQTECVLKPVESSDMKMTQLF TKVESEDTSSLFDKLKKEPDALTLL APAAGDTIISLDFGSNDTETDDQQL EEVPLYNDVMLPSPNEKLQNINLAM SPLPTAETPKPLRSSADPALNQEVA LKLEPNPESLELSFTMPQIQDQTPS PSDGSTRQSSPEPNSPSEYCFYVDS DMVNEFKLELVEKLFAEDTEAKNPF STQDTDLDLEMLAPYIPMDDDFQLR SFDQLSPLESSSASPESASPQSTVT VFQQTQIQEPTANATTTTATTDELK TVTKDRMEDIKILIASPSPTHIHKE TTSATSSPYRDTQSRTASPNRAGKG VIEQTEKSHPRSPNVLSVALSQRTT VPEEELNPKILALQNAQRKRKMEHD GSLFQAVGII
• Purity: Glutathione Sepharose 4 Fast Flow
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Notes: Best use within three months from the date of receipt of this protein.
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.

Gene Information

• Gene Name: HIF1A hypoxia inducible factor 1 subunit alpha [ Homo sapiens (human) ]
• Official Symbol: HIF1A
• Synonyms: HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
• Gene ID: 3091
• mRNA Refseq: NM_181054.1
• Protein Refseq: NP_851397.1
• MIM: 603348
• UniProt ID: Q16665