Recombinant Full Length Human HLA-B Protein, C-Flag-tagged

• Cat.No.: HLA-B-242HFL
• Product Overview: Recombinant Full Length Human HLA-B Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 37.8 kDa
• AA Sequence: MLVMAPRTVLLLLSAALALTETWAG SHSMRYFDTAMSRPGRGEPRFISVG YVDDTQFVRFDSDAASPREE PRAPWIEQEGPEYWDRNTQIFKTNT QTDRESLRNLRGYYNQSEAGSHTLQ SMYGCDVGPDGRLLRGHNQY AYDGKDYIALNEDLRSWTAADTAAQ ITQRKWEAARVAEQDRAYLEGTCVE WLRRYLENGKDTLERADPPK THVTHHPISDHEATLRCWALGFYPA EITLTWQRDGEDQTQDTELVETRPA GDRTFQKWAAVVVPSGEEQR YTCHVQHEGLPKPLTLRWEPSSQST VPIVGIVAGLAVLAVVVIGAVVAAV MCRRKSSGGKGGSYSQAACS
  DSAQGSDVSLTATRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis

Gene Information

• Gene Name: HLA-B major histocompatibility complex, class I, B [ Homo sapiens (human) ]
• Official Symbol: HLA-B
• Synonyms: AS; HLAB; B-4901
• Gene ID: 3106
• mRNA Refseq: NM_005514.8
• Protein Refseq: NP_005505.2
• MIM: 142830
• UniProt ID: P01889