Recombinant Full Length Human HSD3B1 Protein, C-Flag-tagged

• Cat.No.: HSD3B1-1705HFL
• Product Overview: Recombinant Full Length Human HSD3B1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 42.1 kDa
• AA Sequence: MTGWSCLVTGAGGFLGQRIIRLLVK EKELKEIRVLDKAFGPELREEFSKL QNKTKLTVLEGDILDEPFLK RACQDVSVIIHTACIIDVFGVTHRE SIMNVNVKGTQLLLEACVQASVPVF IYTSSIEVAGPNSYKEIIQN GHEEEPLENTWPAPYPHSKKLAEKA VLAANGWNLKNGGTLYTCALRPMYI YGEGSRFLSASINEALNNNG ILSSVGKFSTVNPVYVGNVAWAHIL ALRALQDPKKAPSIRGQFYYISDDT PHQSYDNLNYTLSKEFGLRL DSRWSFPLSLMYWIGFLLEIVSFLL RPIYTYRPPFNRHIVTLSNSVFTFS YKKAQRDLAYKPLYSWEEAK
  QKTVEWVGSLVDRHKENLKSKTQTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Transmembrane
• Protein Pathways: Androgen and estrogen metabolism, C21-Steroid hormone metabolism, Metabolic pathways

Gene Information

• Gene Name: HSD3B1 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 [ Homo sapiens (human) ]
• Official Symbol: HSD3B1
• Synonyms: HSD3B; HSDB3; HSDB3A; SDR11E1; 3BETAHSD
• Gene ID: 3283
• mRNA Refseq: NM_000862.3
• Protein Refseq: NP_000853.1
• MIM: 109715
• UniProt ID: P14060