Recombinant Full Length Human ICA1 Protein, C-Flag-tagged

• Cat.No.: ICA1-845HFL
• Product Overview: Recombinant Full Length Human ICA1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a protein with an arfaptin homology domain that is found both in the cytosol and as membrane-bound form on the Golgi complex and immature secretory granules. This protein is believed to be an autoantigen in insulin-dependent diabetes mellitus and primary Sjogren's syndrome. Several transcript variants encoding two different isoforms have been found for this gene.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 54.5 kDa
• AA Sequence: MSGHKCYPWDLQDRYAQDKSVVNKM QQKYWETKQAFIKATGKKEDEHVVA SDADLDAKLELFHSIQRTCL DLSKAIVLYQKRICFLSQEENELGK FLRSQGFQDKTRAGKMMQATGKALC FSSQQRLALRNPLCRFHQEV ETFRHRAISDTWLTVNRMEQCRTEY RGALLWMKDVSQELDPDLYKQMEKF RKVQTQVRLAKKNFDKLKMD VCQKVDLLGASRCNLLSHMLATYQT TLLHFWEKTSHTMAAIHESFKGYQP YEFTTLKSLQDPMKKLVEKE EKKKINQQESTDAAVQEPSQLISLE EENQRKESSSFKTEDGKSILSALDK GSTHTACSGPIDELLDMKSE EGACLGPVAGTPEPEGADKDDLLLL SEIFNASSLEEGEFSKEWAAVFGDG QVKEPVPTMALGEPDPKAQT
  GSGFLPSQLLDQNMKDLQASLQEPA KAASDLTAWFSLFADLDPLSNPDAV GKTDKEHELLNATRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Type I diabetes mellitus

Gene Information

• Gene Name: ICA1 islet cell autoantigen 1 [ Homo sapiens (human) ]
• Official Symbol: ICA1
• Synonyms: ICA69; ICAp69
• Gene ID: 3382
• mRNA Refseq: NM_004968.4
• Protein Refseq: NP_004959.2
• MIM: 147625
• UniProt ID: Q05084